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Anti-Clostridium botulinum botA/BOTOX Recombinant Nanobody (SAA0936) (JN888163)

Anti-Clostridium botulinum botA/BOTOX Recombinant Nanobody (SAA0936)
Anti-Clostridium botulinum botA/BOTOX Recombinant Nanobody (SAA0936)
Цена(USD):
Спецификация:
  • 20ug
  • 50ug
  • 100ug
  • 1mg
Количество:
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  • Обзор

  • Изображения

  • Ссылки

  • Даташит

  • SDS

Обзор
Каталожный номерJN888163
Описание
Anti-Clostridium botulinum botA/BOTOX Nanobody (SAA0936) [SAA0936] (JN888163) is a alpaca monoclonal antibody detecting BOTOX in ELISA.
Реактивность видовClostridium botulinum
ПримененияELISA
Хозяинский видAlpaca
КлоональностьMonoclonal
ИзотипVHH-8His-Cys-tag
ID клонаSAA0936
Цель Botulinum neurotoxin type A, BoNT/A, Bontoxilysin-A, BOTOX, Botulinum neurotoxin A light chain, LC, 3.4.24.69, Botulinum neurotoxin A heavy chain, HC, botA, bna, CBO0806, CLC_0862
Уровень эндотоксинов Please contact with the lab for this information.
Чистота >95% as determined by SDS-PAGE.
Очистка Purified by Nickel column.
Номер доступа P0DPI1
Форма Liquid
Буфер хранения 0.01M PBS pH 7.4

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта
Применение Разбавление
ELISA 1:2000-1:20000
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон

Botulinum neurotoxin type A (botA/BOTOX) is a ~149 kDa protein. Botulinum toxin causes flaccid paralysis by inhibiting neurotransmitter (acetylcholine) release from the presynaptic membranes of nerve terminals of the eukaryotic host skeletal and autonomic nervous system, with frequent heart or respiratory failure. Precursor of botulinum neurotoxin A which has 2 coreceptors; complex polysialylated gangliosides found on neural tissue and specific membrane-anchored proteins of synaptic vesicles. Receptor proteins are exposed on host presynaptic cell membrane during neurotransmitter release, when the toxin heavy chain (HC) binds to them. Upon synaptic vesicle recycling the toxin is taken up via the endocytic pathway. When the pH of the toxin-containing endosome drops a structural rearrangement occurs so that the N-terminus of the HC forms pores that allows the light chain (LC) to translocate into the cytosol.

1. Blasi, J. et al. (1993) Nature 365, 160-3. PMID: 8103915
2. Fu, Z. et al. (2009) Biochemistry 48, 5631-41. PMID: 19476346
Примечание For research use only.
Изображения
  • Anti-Clostridium botulinum botA/BOTOX Recombinant Nanobody (SAA0936)

    SDS-PAGE

    SDS-PAGE for Anti-Clostridium botulinum botA/BOTOX Nanobody (SAA0936)

Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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