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Anti-Clostridium botulinum botA/BOTOXPolyclonal Antibody (JN888014)

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Aperçu
Numéro de catalogueJN888014
Description
Anti-Clostridium botulinum BotA Polyclonal Antibody (JN888014) is a rabbit polyclonal antibody detecting botA. Suitable for Clostridium botulinum.
Reactivité des espècesClostridium botulinum
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Clostridium botulinum BotA (Met1-Glu424).
Cible BOTOX, BoNT/A, Bontoxilysin-A, Botulinum neurotoxin A heavy chain, Botulinum neurotoxin A light chain, Botulinum neurotoxin type A, Botulinum neurotoxin type A1, EC:3.4.24.69, HC, LC, atx, bonT, botA
Niveau d'endotoxines Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Numéro d'accès P0DPI0
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Contexte

Botulinum neurotoxin type A (BotA) is a ~149 kDa protein. Botulinum toxin causes flaccid paralysis by inhibiting neurotransmitter (acetylcholine) release from the presynaptic membranes of nerve terminals of the eukaryotic host skeletal and autonomic nervous system, with frequent heart or respiratory failure. Precursor of botulinum neurotoxin A which has 2 coreceptors; complex polysialylated gangliosides found on neural tissue and specific membrane-anchored proteins of synaptic vesicles. Receptor proteins are exposed on host presynaptic cell membrane during neurotransmitter release, when the toxin heavy chain (HC) binds to them. Upon synaptic vesicle recycling the toxin is taken up via the endocytic pathway. When the pH of the toxin-containing endosome drops a structural rearrangement occurs so that the N-terminus of the HC forms pores that allows the light chain (LC) to translocate into the cytosol.

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2. Zhou, L. et al. (1995) Biochemistry 34, 15175-81. PMID: 7578132
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8. Dong, M. et al. (2008) Molecular biology of the cell 19, 5226-37. PMID: 18815274
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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