Anti-Clostridium novyi Toxin A/tcdA Polyclonal Antibody (JN055014)

Name: Anti-Clostridium novyi Toxin A/tcdA Polyclonal Antibody
Brand: abinScience
SKU: JN055014
Price: 168 USD
Availability: InStock

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SDS

Aperçu

Numéro de catalogue

JN055014

Description

Anti-Clostridium novyi Toxin A/tcdA Polyclonal Antibody (JN055014) is a rabbit polyclonal antibody detecting Toxin A in ELISA, IHC, WB. Suitable for Clostridium novyi.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactivité des espèces

Clostridium novyi

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Clostridium novyi Toxin A/tcdA (Met1-Glu551).

Cible

Toxin A, 3.4.22.-, N-acetylglucosaminyltransferase TcdA, 2.4.1.-, tcdA, toxA

Purification

Purified by antigen affinity column.

Numéro d'accès

Q46149

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Toxin A (tcdA) is a ~250 kDa protein. Precursor of a cytotoxin, which enters into host cells and mediates autoprocessing to release the active toxin (N-acetylglucosaminyltransferase TcdA) into the host cytosol. Once entered into host cells, acidification in the endosome promotes the membrane insertion of the translocation region and formation of a pore, leading to translocation of the GT44 and peptidase C80 domains across the endosomal membrane. This activates the peptidase C80 domain and autocatalytic processing, releasing the N-terminal part (N-acetylglucosaminyltransferase TcdA), which constitutes the active part of the toxin, in the cytosol.

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1. Reineke, J. et al. (2007) Nature 446, 415-9. PMID: 17334356

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Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-TCDA polyclonal antibody at 1 ug/mL on various samples.
Lane 1: JN055012 100ng

Reférences

Fiche technique

Anti-Clostridium novyi Toxin A/tcdA Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

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