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| カタログ番号 | JN055014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-Clostridium novyi Toxin A/tcdA Polyclonal Antibody (JN055014) is a rabbit polyclonal antibody detecting Toxin A in ELISA, IHC, WB. Suitable for Clostridium novyi.
Highlights
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| 種反応性 | Clostridium novyi | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Clostridium novyi Toxin A/tcdA (Met1-Glu551). | ||||||||
| ターゲット | Toxin A, 3.4.22.-, N-acetylglucosaminyltransferase TcdA, 2.4.1.-, tcdA, toxA | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | Q46149 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Toxin A (tcdA) is a ~250 kDa protein. Precursor of a cytotoxin, which enters into host cells and mediates autoprocessing to release the active toxin (N-acetylglucosaminyltransferase TcdA) into the host cytosol. Once entered into host cells, acidification in the endosome promotes the membrane insertion of the translocation region and formation of a pore, leading to translocation of the GT44 and peptidase C80 domains across the endosomal membrane. This activates the peptidase C80 domain and autocatalytic processing, releasing the N-terminal part (N-acetylglucosaminyltransferase TcdA), which constitutes the active part of the toxin, in the cytosol. _x005F_x005F_x000D_ _x005F_x005F_x000D_1. Reineke, J. et al. (2007) Nature 446, 415-9. PMID: 17334356 _x005F_x005F_x000D_ | ||||||||
| 注意事項 | For research use only. |
Western blot analysis was performed using anti-TCDA polyclonal antibody at 1 ug/mL on various samples.
Lane 1: JN055012 100ng
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