Anti-Clostridium perfringens pfo/Perfringolysin O/Theta-toxin Recombinant Antibody (HS1) (JN963033)

Name: Anti-Clostridium perfringens pfo/Perfringolysin O/Theta-toxin Recombinant Antibody (HS1)
Brand: abinScience
SKU: JN963033
Price: 126 USD
Availability: InStock

Datasheet Compartir producto Copiar información

Precio(USD):

Especificación:

20ug
50ug
100ug
1mg

Número:

Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

JN963033

Reactividad de especies

Clostridium perfringens, Streptococcus dysgalactiae

Aplicaciones

ELISA, Neutralization, WB

Especie huésped

Mouse

Clonalidad

Monoclonal

Isotipo

IgG2a

ID de clon

HS1

Objetivo

Perfringolysin O, PFO, Theta-toxin, Thiol-activated cytolysin, pfo, pfoA, pfoR, CPE0163, Streptolysin O, SLO, Thiol-activated cytolysin, slo, Clostridium perfringens (strain 13 / Type A), Streptococcus dysgalactiae subsp. equisimilis (Streptococcus equisimilis)

Nivel de endotoxinas

Please contact with the lab for this information.

Pureza

>95% as determined by SDS-PAGE.

Purificación

Protein A/G purified from cell culture supernatant.

Número de acceso

P0C2E9, Q54114

RRID

Anti-Clostridium perfringens pfo/Perfringolysin O/Theta-toxin Antibody (HS1) (abinScience Cat# JN963033, RRID:AB_3729019)
_x005F_x005F_x000D_

Forma

Liquid

Buffer de almacenamiento

0.01M PBS pH 7.4

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:2000-1:20000
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Fondo

Perfringolysin O (pfo/Theta-toxin) is a ~55 kDa protein. A cholesterol-dependent toxin that causes cytolysis by forming pores in cholesterol-containing host membranes. After binding to target membranes, the protein assembles into a pre-pore complex. A major conformational change leads to insertion in the host membrane and formation of an oligomeric pore complex. Cholesterol is required for binding to host cell membranes, membrane insertion and pore formation; cholesterol binding is mediated by a Thr-Leu pair in the C-terminus. Can be reversibly inactivated by oxidation.

Nota

For research use only.

Imágenes

Bioactivity

Detects Clostridium perfringens pfo in indirect ELISAs.
SDS-PAGE

SDS-PAGE for Anti-Clostridium perfringens pfo/Perfringolysin O/Theta-toxin Antibody (HS1).
Western blot

Western blot analysis was performed using anti-Theta-toxin monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant Clostridium perfringens pfo/Perfringolysin O/Theta-toxin (Catalog No: JN963012)
Lane 2: negative control

Referencias

Datasheet

Anti-Clostridium perfringens pfo/Perfringolysin O/Theta-toxin Recombinant Antibody (HS1).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo