Anti-Clostridium perfringens pfo/Perfringolysin O/Theta-toxin Recombinant Antibody (HS1) (JN963033)

Name: Anti-Clostridium perfringens pfo/Perfringolysin O/Theta-toxin Recombinant Antibody (HS1)
Brand: abinScience
SKU: JN963033
Price: 126 USD
Availability: InStock

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Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

JN963033

Reactivité des espèces

Clostridium perfringens, Streptococcus dysgalactiae

Applications

ELISA, Neutralization, WB

Espèce hôte

Mouse

Clonalité

Monoclonal

Isotype

IgG2a

ID de clone

HS1

Cible

Perfringolysin O, PFO, Theta-toxin, Thiol-activated cytolysin, pfo, pfoA, pfoR, CPE0163, Streptolysin O, SLO, Thiol-activated cytolysin, slo, Clostridium perfringens (strain 13 / Type A), Streptococcus dysgalactiae subsp. equisimilis (Streptococcus equisimilis)

Niveau d'endotoxines

Please contact with the lab for this information.

Pureté

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Numéro d'accès

P0C2E9, Q54114

RRID

Anti-Clostridium perfringens pfo/Perfringolysin O/Theta-toxin Antibody (HS1) (abinScience Cat# JN963033, RRID:AB_3729019)
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Forme

Liquid

Tampon de stockage

0.01M PBS pH 7.4

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Contexte

Perfringolysin O (pfo/Theta-toxin) is a ~55 kDa protein. A cholesterol-dependent toxin that causes cytolysis by forming pores in cholesterol-containing host membranes. After binding to target membranes, the protein assembles into a pre-pore complex. A major conformational change leads to insertion in the host membrane and formation of an oligomeric pore complex. Cholesterol is required for binding to host cell membranes, membrane insertion and pore formation; cholesterol binding is mediated by a Thr-Leu pair in the C-terminus. Can be reversibly inactivated by oxidation.

Note

For research use only.

Images

Bioactivity

Detects Clostridium perfringens pfo in indirect ELISAs.
SDS-PAGE

SDS-PAGE for Anti-Clostridium perfringens pfo/Perfringolysin O/Theta-toxin Antibody (HS1).
Western blot

Western blot analysis was performed using anti-Theta-toxin monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant Clostridium perfringens pfo/Perfringolysin O/Theta-toxin (Catalog No: JN963012)
Lane 2: negative control

Reférences

Fiche technique

Anti-Clostridium perfringens pfo/Perfringolysin O/Theta-toxin Recombinant Antibody (HS1).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

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