Anti-Clostridium perfringens pfo/Perfringolysin O/Theta-toxin Polyclonal Antibody (JN963014)

Name: Anti-Clostridium perfringens pfo/Perfringolysin O/Theta-toxin Polyclonal Antibody
Brand: abinScience
SKU: JN963014
Price: 168 USD
Availability: InStock

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Precio(USD):

Especificación:

50ug
100ug

Número:

Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

JN963014

Descripción

Anti-Clostridium perfringens pfo/Perfringolysin O/Theta-toxin Polyclonal Antibody (JN963014) is a rabbit polyclonal antibody detecting Theta-toxin in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactividad de especies

Clostridium perfringens

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Clostridium perfringens pfo/Perfringolysin O/Theta-toxin (Asp30-Asn500).

Objetivo

Perfringolysin O, PFO, Theta-toxin, Thiol-activated cytolysin, pfo, pfoA, pfoR, CPE0163

Purificación

Purified by antigen affinity column.

Número de acceso

P0C2E9

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Perfringolysin O (pfo/Theta-toxin) is a ~55 kDa protein. A cholesterol-dependent toxin that causes cytolysis by forming pores in cholesterol-containing host membranes. After binding to target membranes, the protein assembles into a pre-pore complex. A major conformational change leads to insertion in the host membrane and formation of an oligomeric pore complex. Cholesterol is required for binding to host cell membranes, membrane insertion and pore formation; cholesterol binding is mediated by a Thr-Leu pair in the C-terminus. Can be reversibly inactivated by oxidation.

Nota

For research use only.

Imágenes

Western blot

Western blot analysis was performed using anti-PFO polyclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant Clostridium perfringens pfo/Perfringolysin O/Theta-toxin (Catalog No: JN963012)

Referencias

Datasheet