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Anti-CRYZ Polyclonal Antibody (HB380014)

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Anti-CRYZ Polyclonal Antibody
Anti-CRYZ Polyclonal Antibody
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Aperçu
Numéro de catalogueHB380014
Description
Anti-CRYZ Polyclonal Antibody (HB380014) is a rabbit polyclonal antibody detecting Quinone oxidoreductase; 1.6.5.5; NADPH:quinone reductase; Zeta-crystallin; CRYZ in ELISA, IHC, WB. Suitable for Human and Bovine.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesHuman
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant human CRYZ (Met1-Leu329).
Cible Quinone oxidoreductase; 1.6.5.5; NADPH:quinone reductase; Zeta-crystallin; CRYZ
Niveau d'endotoxines Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Numéro d'accès Q08257, O97764
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Zeta-crystallin (CRYZ) is a ~35 kDa protein. Multifunctional crystallin endowed with unrelated mRNA stabilization and enzymatic oxidoreductase activities. Binds (AU)-rich elements (ARE) in the 3'-UTR of target mRNA to enhance their stability. Upon metabolic acidosis, stabilizes the mRNA encoding SLC12A1 a cotransporter involved in transepithelial NH4(+) reabsorption in the medullary thick ascending limb in kidney. In response to acidosis, also participates to the adaptive increase in renal ammoniagenesis by stabilizing the mRNAs of enzymes involved in glutamine catabolism. By the same mechanism, it also regulates the expression of the antiapoptotic protein BCL2.

1. Fernández, MR. et al. (2007) Cellular and molecular life sciences : CMLS 64, 1419-27. PMID: 17497241
2. Lapucci, A. et al. (2010) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 24, 1852-65. PMID: 20103721
Note For research use only
Images
  • Anti-CRYZ Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-CRYZ polyclonal antibody at 1ug/mL on various samples.
    Lane 1: HepG2 cell lysate
    Lane 2: K562 cell lysate
    Lane 3: Jurkat cell lysate
    Lane 4: Raji cell lysate

Reférences
Fiche technique
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-CRYZ Polyclonal Antibody

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