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Anti-CTBS Polyclonal Antibody (HX869014)

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Vista general
Número de catálogoHX869014
Descripción
Anti-CTBS Polyclonal Antibody (HX869014) is a rabbit polyclonal antibody detecting CTBS in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactividad de especiesHuman
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human CTBS (Glu47-Arg385).
Objetivo CTB, Di-N-acetylchitobiase, CTBS
Purificación Purified by antigen affinity column.
Número de acceso Q01459
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Di-N-acetylchitobiase (CTBS) is a ~43 kDa protein. Reducing-end exoglycosidase involved in glycan degradation. Hydrolyzes N,N'-diacetylchitobiose (GlnNAc2) and higher chitin-oligosaccharides (GlcNAc(n)) to monomeric GlcNAc acting on reducing-end residues. During N-linked glycoprotein degradation, it cleaves single GlcNAc molecules uniquely from the reducing end of N,N'-diacetylchitobiose units linking oligosaccharides to proteins, and therefore it functions after cleavage of the Asn-GlcNAc bond and asparagine removal by glycosylasparaginase.

1. Fisher, KJ. et al. (1992) The Journal of biological chemistry 267, 19607-16. PMID: 1527079
2. Park, C. et al. (2005) The Journal of biological chemistry 280, 37204-16. PMID: 16115860
3. Aronson, NN. et al. (2006) Bioscience, biotechnology, and biochemistry 70, 1537-41. PMID: 16794344
Nota For research use only.
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Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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