Anti-CTNNB1 Polyclonal Antibody (HW518014)

Name: Anti-CTNNB1 Polyclonal Antibody
Brand: abinScience
SKU: HW518014
Price: 96 USD
Availability: InStock

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概要
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参考文献
データシート
SDS

概要

カタログ番号

HW518014

説明

Anti-CTNNB1 Polyclonal Antibody (HW518014) is a rabbit polyclonal antibody detecting CTNNB1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

種反応性

Human, Mouse, Rat

アプリケーション

ELISA, IHC, WB

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

免疫原

E. coli - derived recombinant Human CTNNB1 (Met1-Leu781).

ターゲット

CTNNB, Catenin beta-1, CTNNB1, Beta-catenin

エンドトキシンレベル

Please contact with the lab for this information.

精製

Purified by antigen affinity column.

アクセッション番号

P35222, Q02248, Q9WU82

形態

Liquid

保存バッファー

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報

アプリケーション	希釈
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

安定性と保存

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Catenin beta-1 (CTNNB1) is a ~85 kDa protein. Key downstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. Also acts as a coactivator for other transcription factors, such as NR5A2. Promotes epithelial to mesenchymal transition/mesenchymal to epithelial transition (EMT/MET) via driving transcription of CTNNB1/TCF-target genes.

1. Lillehoj, EP. et al. (2007) Biochimica et biophysica acta 1773, 1028-38. PMID: 17524503

2. Weiske, J. et al. (2007) Proceedings of the National Academy of Sciences of the United States of America 104, 20344-9. PMID: 18077326

3. Bahmanyar, S. et al. (2008) Genes & development 22, 91-105. PMID: 18086858

4. Li, H. et al. (2009) The Journal of biological chemistry 284, 2012-22. PMID: 18957423

5. Fiset, A. et al. (2011) Cellular signalling 23, 911-9. PMID: 21262353

6. Satow, R. et al. (2012) Gastroenterology 142, 572-81. PMID: 22155184

7. Genovese, G. et al. (2012) Cell cycle (Georgetown, Tex.) 11, 2206-15. PMID: 22647378

8. Yu, Y. et al. (2012) EMBO reports 13, 750-8. PMID: 22699938

10. Park, J. et al. (2018) Stem cell reports 11, 115-127. PMID: 29910125

注意事項

For research use only.

イメージ

Western blot

Western blot analysis was performed using anti-CTNNB1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: A431 cell lysate
Lane 2: Lncap cell lysate

参考文献

データシート

Anti-CTNNB1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

組換えタンパク質

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アッセイキット

Anti-CTNNB1 Polyclonal Antibody (HW518014)

概要

イメージ

参考文献

データシート

SDS

クイックアクセス

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