Anti-CTNNB1 Polyclonal Antibody (HW518014)

Name: Anti-CTNNB1 Polyclonal Antibody
Brand: abinScience
SKU: HW518014
Price: 96 USD
Availability: InStock

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Каталожный номер

HW518014

Описание

Anti-CTNNB1 Polyclonal Antibody (HW518014) is a rabbit polyclonal antibody detecting CTNNB1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Реактивность видов

Human, Mouse, Rat

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant Human CTNNB1 (Met1-Leu781).

Цель

CTNNB, Catenin beta-1, CTNNB1, Beta-catenin

Уровень эндотоксинов

Please contact with the lab for this information.

Очистка

Purified by antigen affinity column.

Номер доступа

P35222, Q02248, Q9WU82

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

Catenin beta-1 (CTNNB1) is a ~85 kDa protein. Key downstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. Also acts as a coactivator for other transcription factors, such as NR5A2. Promotes epithelial to mesenchymal transition/mesenchymal to epithelial transition (EMT/MET) via driving transcription of CTNNB1/TCF-target genes.

1. Lillehoj, EP. et al. (2007) Biochimica et biophysica acta 1773, 1028-38. PMID: 17524503

2. Weiske, J. et al. (2007) Proceedings of the National Academy of Sciences of the United States of America 104, 20344-9. PMID: 18077326

3. Bahmanyar, S. et al. (2008) Genes & development 22, 91-105. PMID: 18086858

4. Li, H. et al. (2009) The Journal of biological chemistry 284, 2012-22. PMID: 18957423

5. Fiset, A. et al. (2011) Cellular signalling 23, 911-9. PMID: 21262353

6. Satow, R. et al. (2012) Gastroenterology 142, 572-81. PMID: 22155184

7. Genovese, G. et al. (2012) Cell cycle (Georgetown, Tex.) 11, 2206-15. PMID: 22647378

8. Yu, Y. et al. (2012) EMBO reports 13, 750-8. PMID: 22699938

10. Park, J. et al. (2018) Stem cell reports 11, 115-127. PMID: 29910125

Примечание

For research use only.

Изображения

Western blot

Western blot analysis was performed using anti-CTNNB1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: A431 cell lysate
Lane 2: Lncap cell lysate

Ссылки

Даташит

Anti-CTNNB1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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