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Обзор
Каталожный номерHY284014
Описание
Anti-CTSG Polyclonal Antibody (HY284014) is a rabbit polyclonal antibody detecting CTSG in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Реактивность видовHuman
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant Human CTSG (Ile21-Leu255).
Цель Cathepsin G, CG, CTSG
Очистка Purified by antigen affinity column.
Номер доступа P08311
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта
Применение Разбавление
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон

Cathepsin G (CTSG) is a ~28 kDa protein. Serine protease with trypsin- and chymotrypsin-like specificity. Also displays antibacterial activity against Gram-negative and Gram-positive bacteria independent of its protease activity. Prefers Phe and Tyr residues in the P1 position of substrates but also cleaves efficiently after Trp and Leu. Shows a preference for negatively charged amino acids in the P2' position and for aliphatic amino acids both upstream and downstream of the cleavage site. Required for recruitment and activation of platelets which is mediated by the F2RL3/PAR4 platelet receptor.

1. Thorpe, M. et al. (2018) PloS one 13, e0195077. PMID: 29652924
2. Avril, LE. et al. (1994) FEBS letters 345, 81-6. PMID: 8194606
3. Bangalore, N. et al. (1990) The Journal of biological chemistry 265, 13584-8. PMID: 2116408
4. Alford, CE. et al. (1990) Journal of general microbiology 136, 997-100. PMID: 2117044
5. Sambrano, GR. et al. (2000) The Journal of biological chemistry 275, 6819-23. PMID: 10702240
6. Selak, MA. et al. (1988) The Biochemical journal 251, 293-9. PMID: 3390156
7. Hase-Yamazaki, T. et al. (1995) Cellular immunology 160, 24-32. PMID: 7842483
8. Yamazaki, T. et al. (1997) Journal of leukocyte biology 61, 73-9. PMID: 9000539
Примечание For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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