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Anti-CYP11B1 Polyclonal Antibody (HB959014)

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Aperçu
Numéro de catalogueHB959014
Description
Anti-CYP11B1 Polyclonal Antibody (HB959014) is a rabbit polyclonal antibody detecting CYP11B1 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesHuman
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human CYP11B1 (Gly134-Asn503).
Cible CYP11B1, Cytochrome P-450c11, Steroid 11-beta-hydroxylase, CYP11B1, Cytochrome P450C11, CYPXIB1, Cytochrome P450 11B1, mitochondrial, S11BH
Purification Purified by antigen affinity column.
Numéro d'accès P15538
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Cytochrome P450 11B1, mitochondrial (CYP11B1) is a ~57 kDa protein. A cytochrome P450 monooxygenase involved in the biosynthesis of adrenal corticoids. Catalyzes a variety of reactions that are essential for many species, including detoxification, defense, and the formation of endogenous chemicals like steroid hormones. Steroid 11beta, 18- and 19-hydroxylase with preferred regioselectivity at 11beta, then 18, and lastly 19. Catalyzes the hydroxylation of 11-deoxycortisol and 11-deoxycorticosterone (21-hydroxyprogesterone) at 11beta position, yielding cortisol or corticosterone, respectively, but cannot produce aldosterone. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate for hydroxylation and reducing the second into a water molecule.

1. Bureik, M. et al. (2002) Endocrine research 28, 351-5. PMID: 12530636
2. Pascoe, L. et al. (1992) Proceedings of the National Academy of Sciences of the United States of America 89, 8327-31. PMID: 1518866
3. Curnow, KM. et al. (1991) Molecular endocrinology (Baltimore, Md.) 5, 1513-22. PMID: 1775135
4. Zöllner, A. et al. (2008) The FEBS journal 275, 799-810. PMID: 18215163
5. Strushkevich, N. et al. (2013) Molecular endocrinology (Baltimore, Md.) 27, 315-24. PMID: 23322723
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-CYP11B1 Polyclonal Antibody

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