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Anti-CYP26A1 Polyclonal Antibody (HT346014)

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Vista general
Número de catálogoHT346014
Descripción
Anti-CYP26A1 Polyclonal Antibody (HT346014) is a rabbit polyclonal antibody detecting CYP26A1 in ELISA, IHC, WB. Suitable for Human, Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman, Mouse
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human CYP26A1 (Arg73-Ile255).
Objetivo P450RAI1, Cytochrome P450 26A1, Cytochrome P450RAI, hP450RAI, CYP26A1, Cytochrome P450 retinoic acid-inactivating 1, Retinoic acid-metabolizing cytochrome, CYP26, Retinoic acid 4-hydroxylase
Purificación Purified by antigen affinity column.
Número de acceso O43174
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Cytochrome P450 26A1 (CYP26A1) is a ~56 kDa protein. A cytochrome P450 monooxygenase involved in the metabolism of retinoates (RAs), the active metabolites of vitamin A, and critical signaling molecules in animals. RAs exist as at least four different isomers: all-trans-RA (atRA), 9-cis-RA, 13-cis-RA, and 9,13-dicis-RA, where atRA is considered to be the biologically active isomer, although 9-cis-RA and 13-cis-RA also have activity (Probable). Catalyzes the hydroxylation of atRA primarily at C-4 and C-18, thereby contributing to the regulation of atRA homeostasis and signaling. Hydroxylation of atRA limits its biological activity and initiates a degradative process leading to its eventual elimination (Probable). Involved in the convertion of atRA to all-trans-4-oxo-RA.

1. Topletz, AR. et al. (2012) Biochemical pharmacology 83, 149-63. PMID: 22020119
2. White, JA. et al. (1997) The Journal of biological chemistry 272, 18538-41. PMID: 9228017
3. Sonneveld, E. et al. (1998) Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research 9, 629-37. PMID: 9716180
4. Foti, RS. et al. (2016) The Journal of pharmacology and experimental therapeutics 357, 281-92. PMID: 26937021
Nota For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Mass
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Volume
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g/mol
Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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