Anti-DAAM1 Polyclonal Antibody (HV298014)

Name: Anti-DAAM1 Polyclonal Antibody
Brand: abinScience
SKU: HV298014
Price: 96 USD
Availability: InStock

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Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HV298014

Reactivité des espèces

Human, Mouse

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human DAAM1 (Met1-Tyr250).

Cible

KIAA0666, DAAM1, Disheveled-associated activator of morphogenesis 1

Purification

Purified by antigen affinity column.

Numéro d'accès

Q9Y4D1

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Disheveled-associated activator of morphogenesis 1 (DAAM1) is a ~123 kDa protein. Binds to disheveled (Dvl) and Rho, and mediates Wnt-induced Dvl-Rho complex formation. May play a role as a scaffolding protein to recruit Rho-GDP and Rho-GEF, thereby enhancing Rho-GTP formation. Can direct nucleation and elongation of new actin filaments. Involved in building functional cilia. Involved in the organization of the subapical actin network in multiciliated epithelial cells.

1. Aspenström, P. et al. (2006) Experimental cell research 312, 2180-94. PMID: 16630611

2. Lu, J. et al. (2007) Journal of molecular biology 369, 1258-69. PMID: 17482208

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-DAAM1 polyclonal antibody at 1μg/ml on various samples.
Lane 1: Hela cell lysate
Lane 2: HepG2 cell lysate
Lane 3: Jurkat cell lysate
Lane 4: Mouse liver lysate
Lane 5: Mouse brain lysate
Lane 6: Mouse heart lysate

Reférences

Fiche technique

Anti-DAAM1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

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