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Anti-DAZAP2 Polyclonal Antibody (HB402014)

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Vista general
Número de catálogoHB402014
Descripción
Anti-DAZAP2 Polyclonal Antibody (HB402014) is a rabbit polyclonal antibody detecting DAZ-associated protein 2; Deleted in azoospermia-associated protein 2; Proline-rich transcript in brain protein; DAZAP2; KIAA0058; PRTB in ELISA, IHC, WB. Suitable for Human, Bovine, Mouse, and Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman, Mouse, Rat
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant human DAZAP2 (Ala41-Trp168).
Objetivo DAZ-associated protein 2; Deleted in azoospermia-associated protein 2; Proline-rich transcript in brain protein; DAZAP2; KIAA0058; PRTB
Nivel de endotoxinas Please contact with the lab for this information.
Purificación Purified by antigen affinity column.
Número de acceso Q15038, Q3T0K9, Q9DCP9, P60486
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

DAZ-associated protein 2 (DAZAP2) is a ~17 kDa protein. In unstressed cells, promotes SIAH1-mediated polyubiquitination and degradation of the serine/threonine-protein kinase HIPK2, probably by acting as a loading factor that potentiates complex formation between HIPK2 and ubiquitin ligase SIAH1. In response to DNA damage, localizes to the nucleus following phosphorylation by HIPK2 and modulates the expression of a subset of TP53/p53 target genes by binding to TP53 at target gene promoters. This limits the expression of a number of cell death-mediating TP53 target genes, reducing DNA damage-induced cell death. Enhances the binding of transcription factor TCF7L2/TCF4, a Wnt signaling pathway effector, to the promoters of target genes. Plays a role in stress granule formation.

1. Liebl, MC. et al. (2021) Nucleic acids research 49, 2759-2776. PMID: 33591310
2. Kim, JE. et al. (2008) Molecular and cellular biology 28, 803-13. PMID: 17984221
Nota For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-DAZAP2 Polyclonal Antibody

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