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Anti-DAZAP2 Polyclonal Antibody (HB402014)

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Aperçu
Numéro de catalogueHB402014
Description
Anti-DAZAP2 Polyclonal Antibody (HB402014) is a rabbit polyclonal antibody detecting DAZ-associated protein 2; Deleted in azoospermia-associated protein 2; Proline-rich transcript in brain protein; DAZAP2; KIAA0058; PRTB in ELISA, IHC, WB. Suitable for Human, Bovine, Mouse, and Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant human DAZAP2 (Ala41-Trp168).
Cible DAZ-associated protein 2; Deleted in azoospermia-associated protein 2; Proline-rich transcript in brain protein; DAZAP2; KIAA0058; PRTB
Niveau d'endotoxines Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Numéro d'accès Q15038, Q3T0K9, Q9DCP9, P60486
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

DAZ-associated protein 2 (DAZAP2) is a ~17 kDa protein. In unstressed cells, promotes SIAH1-mediated polyubiquitination and degradation of the serine/threonine-protein kinase HIPK2, probably by acting as a loading factor that potentiates complex formation between HIPK2 and ubiquitin ligase SIAH1. In response to DNA damage, localizes to the nucleus following phosphorylation by HIPK2 and modulates the expression of a subset of TP53/p53 target genes by binding to TP53 at target gene promoters. This limits the expression of a number of cell death-mediating TP53 target genes, reducing DNA damage-induced cell death. Enhances the binding of transcription factor TCF7L2/TCF4, a Wnt signaling pathway effector, to the promoters of target genes. Plays a role in stress granule formation.

1. Liebl, MC. et al. (2021) Nucleic acids research 49, 2759-2776. PMID: 33591310
2. Kim, JE. et al. (2008) Molecular and cellular biology 28, 803-13. PMID: 17984221
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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C₁ × V₁ = C₂ × V₂
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C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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Anti-DAZAP2 Polyclonal Antibody

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