Anti-DBH Polyclonal Antibody (HY186014)

Name: Anti-DBH Polyclonal Antibody
Brand: abinScience
SKU: HY186014
Price: 168 USD
Availability: InStock

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50ug
100ug

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Aperçu
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Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HY186014

Description

Anti-DBH Polyclonal Antibody (HY186014) is a rabbit polyclonal antibody detecting DBH in ELISA, IHC, WB. Suitable for Human, Bovine, and Equus caballus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactivité des espèces

Human

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human DBH (His335-Val571).

Cible

Dopamine beta-hydroxylase, DBH, Dopamine beta-monooxygenase

Purification

Purified by antigen affinity column.

Numéro d'accès

P09172

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Dopamine beta-hydroxylase (DBH) is a ~69 kDa protein. Catalyzes the hydroxylation of dopamine to noradrenaline (also known as norepinephrine), and is thus vital for regulation of these neurotransmitters.

Note

For research use only.

Images

Immunofluorescence

Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting DBH (HY186014) at a concentration of 11.6 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of DBH was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).

Reférences

Fiche technique

Anti-DBH Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

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