Anti-DDOST Polyclonal Antibody (HW572014)

Name: Anti-DDOST Polyclonal Antibody
Brand: abinScience
SKU: HW572014
Price: 168 USD
Availability: InStock

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Обзор

Каталожный номер

HW572014

Описание

Anti-DDOST Polyclonal Antibody (HW572014) is a rabbit polyclonal antibody detecting DDOST in ELISA, IHC, WB. Suitable for Human, Mouse, Dog, Rat, and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Реактивность видов

Human, Mouse, Rat

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant Human DDOST (Ser43-Pro427).

Цель

DDOST 48 kDa subunit, OST48, Oligosaccharyl transferase 48 kDa subunit, KIAA0115, DDOST, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit

Очистка

Purified by antigen affinity column.

Номер доступа

P39656

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit (DDOST) is a ~50 kDa protein. Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity. Required for the assembly of both SST3A- and SS3B-containing OST complexes.

1. Ramírez, AS. et al. (2019) Science (New York, N.Y.) 366, 1372-1375. PMID: 31831667

2. Roboti, P. et al. (2012) Journal of cell science 125, 3474-84. PMID: 22467853

Примечание

For research use only.

Изображения

Ссылки

Даташит

Anti-DDOST Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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