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Anti-DDX6 Polyclonal Antibody (HA609014)

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Vista general
Número de catálogoHA609014
Descripción
Anti-DDX6 Polyclonal Antibody (HA609014) is a rabbit polyclonal antibody detecting Probable ATP-dependent RNA helicase DDX6; 3.6.4.13; ATP-dependent RNA helicase p54; DEAD box protein 6; Oncogene RCK; DDX6; HLR2; RCK in ELISA, IHC, WB. Suitable for Human, Chicken, Mouse, and Frog.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman, Mouse
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant human DDX6 (Glu97-Pro483).
Objetivo Probable ATP-dependent RNA helicase DDX6; 3.6.4.13; ATP-dependent RNA helicase p54; DEAD box protein 6; Oncogene RCK; DDX6; HLR2; RCK
Nivel de endotoxinas Please contact with the lab for this information.
Purificación Purified by antigen affinity column.
Número de acceso P26196, Q5ZKB9, P54823, P54824
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Probable ATP-dependent RNA helicase DDX6 is a ~54 kDa protein. Essential for the formation of P-bodies, cytosolic membrane-less ribonucleoprotein granules involved in RNA metabolism through the coordinated storage of mRNAs encoding regulatory functions. Plays a role in P-bodies to coordinate the storage of translationally inactive mRNAs in the cytoplasm and prevent their degradation. In the process of mRNA degradation, plays a role in mRNA decapping. Blocks autophagy in nutrient-rich conditions by repressing the expression of ATG-related genes through degradation of their transcripts.

1. Ayache, J. et al. (2015) Molecular biology of the cell 26, 2579-95. PMID: 25995375
2. Kamenska, A. et al. (2016) Nucleic acids research 44, 6318-34. PMID: 27342281
3. Balak, C. et al. (2019) American journal of human genetics 105, 509-525. PMID: 31422817
4. Fenger-Grøn, M. et al. (2005) Molecular cell 20, 905-15. PMID: 16364915
5. Hu, G. et al. (2015) Nature cell biology 17, 930-942. PMID: 26098573
Nota For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-DDX6 Polyclonal Antibody

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