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Anti-DGKE Polyclonal Antibody (HW773014)

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Vista general
Número de catálogoHW773014
Descripción
Anti-DGKE Polyclonal Antibody (HW773014) is a rabbit polyclonal antibody detecting DGKE in ELISA, IHC, WB. Suitable for Human and Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactividad de especiesHuman, Mouse
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human DGKE (Leu243-Gly502).
Objetivo DGKE, Diglyceride kinase epsilon, DAG kinase epsilon, DGK-epsilon, Diacylglycerol kinase epsilon, DAGK5
Purificación Purified by antigen affinity column.
Número de acceso P52429
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Diacylglycerol kinase epsilon (DGKE) is a ~63 kDa protein. Membrane-bound diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids. Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes. Also plays an important role in the biosynthesis of complex lipids. Displays specificity for diacylglycerol substrates with an arachidonoyl acyl chain at the sn-2 position, with the highest activity toward 1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycerol the main diacylglycerol intermediate within the phosphatidylinositol turnover cycle. Can also phosphorylate diacylglycerol substrates with a linoleoyl acyl chain at the sn-2 position but much less efficiently.

1. Epand, RM. et al. (2004) Biochemistry 43, 14778-83. PMID: 15544348
2. Lung, M. et al. (2009) The Journal of biological chemistry 284, 31062-73. PMID: 19744926
3. Shulga, YV. et al. (2011) Journal of molecular biology 409, 101-12. PMID: 21477596
4. Shulga, YV. et al. (2011) FEBS letters 585, 4025-8. PMID: 22108654
5. Sato, M. et al. (2013) Pharmacology 92, 99-107. PMID: 23949095
6. Tang, W. et al. (1996) The Journal of biological chemistry 271, 10237-41. PMID: 8626589
Nota For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-DGKE Polyclonal Antibody

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