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Anti-DHFR Polyclonal Antibody (HF957014)

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Vista general
Número de catálogoHF957014
Descripción
Anti-DHFR Polyclonal Antibody (HF957014) is a rabbit polyclonal antibody detecting DHFR in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Pig.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human DHFR (Met1-Asp187).
Objetivo Dihydrofolate reductase, DHFR
Purificación Purified by antigen affinity column.
Número de acceso P00374
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Dihydrofolate reductase (DHFR) is a ~21 kDa protein. Catalyzes the reduction of 7,8-dihydrofolate (DHF) to 5,6,7,8-tetrahydrofolate in a NADPH-dependent manner. Key enzyme in folate metabolism. Contributes to the nuclear and mitochondrial de novo thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFR2.

1. Klon, AE. et al. (2002) Journal of molecular biology 320, 677-93. PMID: 12096917
2. Cody, V. et al. (2004) Acta crystallographica. Section D, Biological crystallography 60, 646-55. PMID: 15039552
3. Reynolds, RC. et al. (2007) Journal of medicinal chemistry 50, 3283-9. PMID: 17569517
4. Cody, V. et al. (2009) Biochemistry 48, 1702-11. PMID: 19196009
5. Volpato, JP. et al. (2009) The Journal of biological chemistry 284, 20079-89. PMID: 19478082
6. McEntee, G. et al. (2011) Proceedings of the National Academy of Sciences of the United States of America 108, 15157-62. PMID: 21876184
7. Gangjee, A. et al. (1998) Journal of medicinal chemistry 41, 3426-34. PMID: 9719595
8. Anderson, DD. et al. (2011) Proceedings of the National Academy of Sciences of the United States of America 108, 15163-8. PMID: 21876188
9. Anderson, DD. et al. (2012) The Journal of biological chemistry 287, 7051-62. PMID: 22235121
Nota For research use only.
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Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-DHFR Polyclonal Antibody

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