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Anti-DHFR Polyclonal Antibody (HF957014)

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Aperçu
Numéro de catalogueHF957014
Description
Anti-DHFR Polyclonal Antibody (HF957014) is a rabbit polyclonal antibody detecting DHFR in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Pig.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesHuman
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human DHFR (Met1-Asp187).
Cible Dihydrofolate reductase, DHFR
Purification Purified by antigen affinity column.
Numéro d'accès P00374
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Dihydrofolate reductase (DHFR) is a ~21 kDa protein. Catalyzes the reduction of 7,8-dihydrofolate (DHF) to 5,6,7,8-tetrahydrofolate in a NADPH-dependent manner. Key enzyme in folate metabolism. Contributes to the nuclear and mitochondrial de novo thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFR2.

1. Klon, AE. et al. (2002) Journal of molecular biology 320, 677-93. PMID: 12096917
2. Cody, V. et al. (2004) Acta crystallographica. Section D, Biological crystallography 60, 646-55. PMID: 15039552
3. Reynolds, RC. et al. (2007) Journal of medicinal chemistry 50, 3283-9. PMID: 17569517
4. Cody, V. et al. (2009) Biochemistry 48, 1702-11. PMID: 19196009
5. Volpato, JP. et al. (2009) The Journal of biological chemistry 284, 20079-89. PMID: 19478082
6. McEntee, G. et al. (2011) Proceedings of the National Academy of Sciences of the United States of America 108, 15157-62. PMID: 21876184
7. Gangjee, A. et al. (1998) Journal of medicinal chemistry 41, 3426-34. PMID: 9719595
8. Anderson, DD. et al. (2011) Proceedings of the National Academy of Sciences of the United States of America 108, 15163-8. PMID: 21876188
9. Anderson, DD. et al. (2012) The Journal of biological chemistry 287, 7051-62. PMID: 22235121
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-DHFR Polyclonal Antibody

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