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Обзор
Каталожный номерHT055014
Описание
Anti-DIAPH1 Polyclonal Antibody (HT055014) is a rabbit polyclonal antibody detecting DIAPH1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Реактивность видовHuman, Mouse
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant Human DIAPH1 (Asp389-Pro583).
Цель Protein diaphanous homolog 1, DIAP1, DRF1, Diaphanous-related formin-1, DIAPH1
Уровень эндотоксинов Please contact with the lab for this information.
Очистка Purified by antigen affinity column.
Номер доступа O60610
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта
Применение Разбавление
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон

Protein diaphanous homolog 1 (DIAPH1) is a ~141 kDa protein. Actin nucleation and elongation factor required for the assembly of F-actin structures, such as actin cables and stress fibers. Binds to the barbed end of the actin filament and slows down actin polymerization and depolymerization. Required for cytokinesis, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics. Functions as a scaffold protein for MAPRE1 and APC to stabilize microtubules and promote cell migration.

1. Zaoui, K. et al. (2010) Proceedings of the National Academy of Sciences of the United States of America 107, 18517-22. PMID: 20937854
2. Bai, SW. et al. (2011) BMC biology 9, 54. PMID: 21834987
3. Stritt, S. et al. (2016) Blood 127, 2903-14. PMID: 26912466
Примечание For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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