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| カタログ番号 | HY587014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-DLAT Polyclonal Antibody (HY587014) is a rabbit polyclonal antibody detecting DLAT/PDC-E2 in ELISA, IHC, WB. Suitable for Human, Mouse.
Highlights
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| 種反応性 | Human, Mouse | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human DLAT (Gln374-Trp631). | ||||||||
| ターゲット | DLTA, M2 antigen complex 70 kDa subunit, 70 kDa mitochondrial autoantigen of primary biliary cirrhosis, Pyruvate dehydrogenase complex component E2, PDC-E2, DLAT, PBC, Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex, Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial, PDCE2 | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | P10515 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial (DLAT/PDC-E2) is a ~68 kDa protein. The pyruvate dehydrogenase (PDH) complex, catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links cytoplasmic glycolysis and the mitochondrial tricarboxylic acid (TCA) cycle (Probable). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and dihydrolipoamide dehydrogenase (E3); (Probable). Within this complex, the catalytic function of this enzyme is to accept, and to transfer to coenzyme A, acetyl groups from acetyl-lipoyl moiety generated by the pyruvate dehydrogenase, leading to acetyl-CoA formation (Probable). | ||||||||
| 注意事項 | For research use only. |
Western blot analysis was performed using anti-DLAT polyclonal antibody at 1μg/ml on various samples.
Lane 1: Hela cell lysate
Lane 2: MCF-7 cell lysate
Lane 3: Lncap cell lysate
Lane 4: K562 cell lysate
Lane 5: HCT116 cell lysate
Lane 6: NIH3T3 cell lysate
Lane 7: Mouse brain lysate
Lane 8: Mouse liver lysate
Lane 9: Mouse kidney lysate
Lane 10: Rat brain lysate
Lane11: Rat liver lysate
Lane12: Rat kidney lysate
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