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Anti-DNASE1 Polyclonal Antibody (HB712014)

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Aperçu
Numéro de catalogueHB712014
Description
Anti-DNASE1 Polyclonal Antibody (HB712014) is a rabbit polyclonal antibody detecting DNASE1 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesHuman
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human DNASE1 (Gly19-Ala259).
Cible DNL1, Deoxyribonuclease I, DNASE1, Dornase alfa, Deoxyribonuclease-1, DNase I, DRNI
Purification Purified by antigen affinity column.
Numéro d'accès P24855
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Deoxyribonuclease-1 (DNASE1) is a ~31 kDa protein. Serum endocuclease secreted into body fluids by a wide variety of exocrine and endocrine organs. Expressed by non-hematopoietic tissues and preferentially cleaves protein-free DNA. Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization. Together with DNASE1L3, plays a key role in degrading neutrophil extracellular traps (NETs).

1. Oliveri, M. et al. (2001) European journal of immunology 31, 743-51. PMID: 11241278
2. Shak, S. et al. (1990) Proceedings of the National Academy of Sciences of the United States of America 87, 9188-92. PMID: 2251263
3. Yasuda, T. et al. (1990) Journal of biochemistry 108, 393-8. PMID: 2277032
Note For research use only.
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Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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