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Anti-DNASE1L3 Polyclonal Antibody (HB541014)

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Vista general
Número de catálogoHB541014
Descripción
Anti-DNASE1L3 Polyclonal Antibody (HB541014) is a rabbit polyclonal antibody detecting Deoxyribonuclease gamma; DNase gamma; 3.1.21.-; DNase I homolog protein DHP2; Deoxyribonuclease I-like 3; DNase I-like 3; Liver and spleen DNase; LS-DNase; LSD; DNASE1L3; DHP2; DNAS1L3 in ELISA, IHC, WB. Suitable for Human and Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactividad de especiesHuman
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant human DNASE1L3 (Met21-Ser305).
Objetivo Deoxyribonuclease gamma; DNase gamma; 3.1.21.-; DNase I homolog protein DHP2; Deoxyribonuclease I-like 3; DNase I-like 3; Liver and spleen DNase; LS-DNase; LSD; DNASE1L3; DHP2; DNAS1L3
Nivel de endotoxinas Please contact with the lab for this information.
Purificación Purified by antigen affinity column.
Número de acceso Q13609, O89107
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Deoxyribonuclease gamma (DNASE1L3) is a ~35 kDa protein. Has DNA hydrolytic activity. Is capable of both single- and double-stranded DNA cleavage, producing DNA fragments with 3'-OH ends. Can cleave chromatin to nucleosomal units and cleaves nucleosomal and liposome-coated DNA. Acts in internucleosomal DNA fragmentation (INDF) during apoptosis and necrosis. The role in apoptosis includes myogenic and neuronal differentiation, and BCR-mediated clonal deletion of self-reactive B cells.

1. Yakovlev, AG. et al. (2000) The Journal of biological chemistry 275, 21302-8. PMID: 10807908
2. Shiokawa, D. et al. (1998) Apoptosis : an international journal on programmed cell death 3, 89-95. PMID: 14646506
3. Sisirak, V. et al. (2016) Cell 166, 88-101. PMID: 27293190
4. Zeng, Z. et al. (1997) Biochemical and biophysical research communications 231, 499-504. PMID: 9070308
5. Baron, WF. et al. (1998) Gene 215, 291-301. PMID: 9714828
6. Errami, Y. et al. (2013) The Journal of biological chemistry 288, 3460-8. PMID: 23229555
7. Mizuta, R. et al. (2013) PloS one 8, e80223. PMID: 24312463
Nota For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-DNASE1L3 Polyclonal Antibody

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