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Anti-DNASE1L3 Polyclonal Antibody (HB541014)

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Aperçu
Numéro de catalogueHB541014
Description
Anti-DNASE1L3 Polyclonal Antibody (HB541014) is a rabbit polyclonal antibody detecting Deoxyribonuclease gamma; DNase gamma; 3.1.21.-; DNase I homolog protein DHP2; Deoxyribonuclease I-like 3; DNase I-like 3; Liver and spleen DNase; LS-DNase; LSD; DNASE1L3; DHP2; DNAS1L3 in ELISA, IHC, WB. Suitable for Human and Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesHuman
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant human DNASE1L3 (Met21-Ser305).
Cible Deoxyribonuclease gamma; DNase gamma; 3.1.21.-; DNase I homolog protein DHP2; Deoxyribonuclease I-like 3; DNase I-like 3; Liver and spleen DNase; LS-DNase; LSD; DNASE1L3; DHP2; DNAS1L3
Niveau d'endotoxines Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Numéro d'accès Q13609, O89107
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Deoxyribonuclease gamma (DNASE1L3) is a ~35 kDa protein. Has DNA hydrolytic activity. Is capable of both single- and double-stranded DNA cleavage, producing DNA fragments with 3'-OH ends. Can cleave chromatin to nucleosomal units and cleaves nucleosomal and liposome-coated DNA. Acts in internucleosomal DNA fragmentation (INDF) during apoptosis and necrosis. The role in apoptosis includes myogenic and neuronal differentiation, and BCR-mediated clonal deletion of self-reactive B cells.

1. Yakovlev, AG. et al. (2000) The Journal of biological chemistry 275, 21302-8. PMID: 10807908
2. Shiokawa, D. et al. (1998) Apoptosis : an international journal on programmed cell death 3, 89-95. PMID: 14646506
3. Sisirak, V. et al. (2016) Cell 166, 88-101. PMID: 27293190
4. Zeng, Z. et al. (1997) Biochemical and biophysical research communications 231, 499-504. PMID: 9070308
5. Baron, WF. et al. (1998) Gene 215, 291-301. PMID: 9714828
6. Errami, Y. et al. (2013) The Journal of biological chemistry 288, 3460-8. PMID: 23229555
7. Mizuta, R. et al. (2013) PloS one 8, e80223. PMID: 24312463
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-DNASE1L3 Polyclonal Antibody

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