中文
English
한국어
日本語
Español
Français
Русский
| カタログ番号 | HW618014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-DNM2 Polyclonal Antibody (HW618014) is a rabbit polyclonal antibody detecting DNM2 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.
Highlights
|
||||||||
| 種反応性 | Human, Mouse, Rat | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human DNM2 (Met1-Gly230). | ||||||||
| ターゲット | DNM2, Dynamin-2, DYN2 | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | P50570 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
||||||||
| 製品使用情報 |
| ||||||||
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Dynamin-2 (DNM2) is a ~98 kDa protein. Catalyzes the hydrolysis of GTP and utilizes this energy to mediate vesicle scission at plasma membrane during endocytosis and filament remodeling at many actin structures during organization of the actin cytoskeleton. Plays an important role in vesicular trafficking processes, namely clathrin-mediated endocytosis (CME), exocytic and clathrin-coated vesicle from the trans-Golgi network, and PDGF stimulated macropinocytosis. During vesicular trafficking process, associates to the membrane, through lipid binding, and self-assembles into ring-like structure through oligomerization to form a helical polymer around the vesicle membrane and leading to vesicle scission. Plays a role in organization of the actin cytoskeleton by mediating arrangement of stress fibers and actin bundles in podocytes. During organization of the actin cytoskeleton, self-assembles into ring-like structure that directly bundles actin filaments to form typical membrane tubules decorated with dynamin spiral polymers. 1. Züchner, S. et al. (2005) Nature genetics 37, 289-94. PMID: 15731758 2. Mooren, OL. et al. (2009) The Journal of biological chemistry 284, 23995-4005. PMID: 19605363 3. Bitoun, M. et al. (2009) Human mutation 30, 1419-27. PMID: 19623537 4. Yeung, ML. et al. (2021) Cell 184, 2212-2228.e12. PMID: 33713620 5. Tassin, TC. et al. (2021) Frontiers in cellular neuroscience 15, 745940. PMID: 34744632 6. Fabrizi, GM. et al. (2007) Neurology 69, 291-5. PMID: 17636067 8. Søreng, K. et al. (2018) EMBO reports 19. PMID: 29437695 | ||||||||
| 注意事項 | For research use only. |
Western blot analysis was performed using anti-DNM2 polyclonal antibody at 1µg/ml on various samples.
Lane 1: Mouse brain lysate
Lane 2: Rat brain lysate
お問い合わせ
+86-027-65523339
中国武漢市深敦寺路666号C棟、武漢、430206
