Anti-DPP9 Polyclonal Antibody (HV555014)

Name: Anti-DPP9 Polyclonal Antibody
Brand: abinScience
SKU: HV555014
Price: 168 USD
Availability: InStock

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Обзор
Изображения
Ссылки
Даташит
SDS

Обзор

Каталожный номер

HV555014

Реактивность видов

Human, Mouse

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant Human DPP9 (Ser36-Ser202).

Цель

Dipeptidyl peptidase 9, Dipeptidyl peptidase IX, DPRP-2, DPLP9, Dipeptidyl peptidase IV-related protein 2, DPP IX, DPP9, Dipeptidyl peptidase-like protein 9, DP9, DPRP2

Очистка

Purified by antigen affinity column.

Номер доступа

Q86TI2

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

Dipeptidyl peptidase 9 (DPP9) is a ~98 kDa protein. Dipeptidyl peptidase that cleaves off N-terminal dipeptides from proteins having a Pro or Ala residue at position 2. Acts as a key inhibitor of caspase-1-dependent monocyte and macrophage pyroptosis in resting cells by preventing activation of NLRP1 and CARD8. Sequesters the cleaved C-terminal part of NLRP1 and CARD8, which respectively constitute the active part of the NLRP1 and CARD8 inflammasomes, in a ternary complex, thereby preventing their oligomerization and activation. The dipeptidyl peptidase activity is required to suppress NLRP1 and CARD8; however, neither NLRP1 nor CARD8 are bona fide substrates of DPP9, suggesting the existence of substrate(s) required for NLRP1 and CARD8 inhibition.

1. Qi, SY. et al. (2003) The Biochemical journal 373, 179-89. PMID: 12662155

2. Bjelke, JR. et al. (2006) The Biochemical journal 396, 391-9. PMID: 16475979

3. Geiss-Friedlander, R. et al. (2009) The Journal of biological chemistry 284, 27211-9. PMID: 19667070

4. Ross, B. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, E1437-E1445. PMID: 29382749

5. Zhong, FL. et al. (2018) The Journal of biological chemistry 293, 18864-18878. PMID: 30291141

6. Huang, M. et al. (2021) Nature 592, 773-777. PMID: 33731929

7. Harapas, CR. et al. (2022) Science immunology 7, eabi4611. PMID: 36112693

8. Okondo, MC. et al. (2017) Nature chemical biology 13, 46-53. PMID: 27820798

9. Johnson, DC. et al. (2018) Nature medicine 24, 1151-1156. PMID: 29967349

Примечание

For research use only.

Изображения

Ссылки

Даташит

Anti-DPP9 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Рекомбинантные Белки

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