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Anti-DUSP11 Polyclonal Antibody (HF776014)

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Anti-DUSP11 Polyclonal Antibody
Anti-DUSP11 Polyclonal Antibody
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Aperçu
Numéro de catalogueHF776014
Description
Anti-DUSP11 Polyclonal Antibody (HF776014) is a rabbit polyclonal antibody detecting DUSP11 in ELISA, IHC, WB. Suitable for Human and Bovine.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesHuman
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human DUSP11 (Gly75-Trp255).
Cible Phosphatase that interacts with RNA/RNP complex 1, Dual specificity protein phosphatase 11, DUSP11, RNA/RNP complex-1-interacting phosphatase, PIR1
Niveau d'endotoxines Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Numéro d'accès O75319
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

RNA/RNP complex-1-interacting phosphatase (DUSP11) is a ~38 kDa protein. Possesses RNA 5'-triphosphatase and diphosphatase activities, but displays a poor protein-tyrosine phosphatase activity. In addition, has phosphatase activity with ATP, ADP and O-methylfluorescein phosphate (in vitro). Binds to RNA. May participate in nuclear mRNA metabolism.

Note For research use only.
Images
  • Anti-DUSP11 Polyclonal Antibody

    Immunofluorescence

    Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting DUSP11 (HF776014) at a concentration of 38.4 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of DUSP11 was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).

Reférences
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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