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| カタログ番号 | JN145014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-E. coli groEL Polyclonal Antibody (JN145014) is a rabbit polyclonal antibody detecting GroEL protein. Suitable for strain K12.
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| 種反応性 | Escherichia coli | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant E. coli groEL (Met1-Met548). | ||||||||
| ターゲット | 60 kDa chaperonin, Chaperonin GroEL, Chaperonin-60, Cpn60, EC:5.6.1.7, GroEL protein, groEL, groL, mopA | ||||||||
| エンドトキシンレベル | Please contact with the lab for this information. | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | P0A6F5 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 再構成 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. | ||||||||
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. | ||||||||
| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Chaperonin GroEL is a ~57 kDa protein. Together with its co-chaperonin GroES, plays an essential role in assisting protein folding. The GroEL-GroES system forms a nano-cage that allows encapsulation of the non-native substrate proteins and provides a physical environment optimized to promote and accelerate protein folding, probably by preventing aggregation and by entropically destabilizing folding intermediates. Rapid binding of ATP, followed by slower binding of the non-native substrate protein and GroES to the cis open ring of GroEL initiates productive folding of the non-native protein inside a highly stable GroEL-ATP-GroES complex. Binding of ATP and GroES induces conformational changes that result in the release of the substrate protein into a nano-cage compartment, within the GroEL central cavity, for folding in isolation. To discharge GroES and substrate protein, ATP hydrolysis in the cis ring is required to form a GroEL-ADP-GroES complex with decreased stability. 1. Goloubinoff, P. et al. (1989) Nature 342, 884-9. PMID: 10532860 2. Tang, YC. et al. (2006) Cell 125, 903-14. PMID: 16751100 3. Martin, J. et al. (1991) Nature 352, 36-42. PMID: 1676490 4. Tang, YC. et al. (2008) The EMBO journal 27, 1458-68. PMID: 18418386 5. Apetri, AC. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 17351-5. PMID: 18987317 6. Chakraborty, K. et al. (2010) Cell 142, 112-22. PMID: 20603018 7. Gupta, AJ. et al. (2014) Journal of molecular biology 426, 2739-54. PMID: 24816391 8. Kusukawa, N. et al. (1989) The EMBO journal 8, 3517-21. PMID: 2573517 9. Hemmingsen, SM. et al. (1988) Nature 333, 330-4. PMID: 2897629 10. Horwich, AL. et al. (1993) Cell 74, 909-17. PMID: 8104102 | ||||||||
| 注意事項 | For research use only |
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