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Anti-E. coli groES Polyclonal Antibody (JN912014)

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Vista general
Número de catálogoJN912014
Descripción
Anti-E. coli groES Polyclonal Antibody (JN912014) is a rabbit polyclonal antibody detecting groES. Suitable for strain K12.
Reactividad de especiesEscherichia coli
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant E. coli groES (Met1-Ala97).
Objetivo 10 kDa chaperonin, Chaperonin-10, Co-chaperonin GroES, Cpn10, groES, groS, mopB
Nivel de endotoxinas Please contact with the lab for this information.
Purificación Purified by antigen affinity column.
Número de acceso P0A6F9
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Envío In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Co-chaperonin GroES is a ~10 kDa protein. Together with the chaperonin GroEL, plays an essential role in assisting protein folding. The GroEL-GroES system forms a nano-cage that allows encapsulation of the non-native substrate proteins and provides a physical environment optimized to promote and accelerate protein folding, probably by preventing aggregation and by entropically destabilizing folding intermediates. GroES binds to the apical surface of the GroEL ring, thereby capping the opening of the GroEL channel. Regulates the ATPase activity of GroEL.

1. Goloubinoff, P. et al. (1989) Nature 342, 884-9. PMID: 10532860
2. Tang, YC. et al. (2006) Cell 125, 903-14. PMID: 16751100
3. Martin, J. et al. (1991) Nature 352, 36-42. PMID: 1676490
4. Tang, YC. et al. (2008) The EMBO journal 27, 1458-68. PMID: 18418386
5. Apetri, AC. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 17351-5. PMID: 18987317
6. Chakraborty, K. et al. (2010) Cell 142, 112-22. PMID: 20603018
7. Gupta, AJ. et al. (2014) Journal of molecular biology 426, 2739-54. PMID: 24816391
8. Kusukawa, N. et al. (1989) The EMBO journal 8, 3517-21. PMID: 2573517
9. Hemmingsen, SM. et al. (1988) Nature 333, 330-4. PMID: 2897629
Nota For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-E. coli groES Polyclonal Antibody

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