Anti-EBF1 Polyclonal Antibody (HV735014)

Name: Anti-EBF1 Polyclonal Antibody
Brand: abinScience
SKU: HV735014
Price: 168 USD
Availability: InStock

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Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HV735014

Description

Anti-EBF1 Polyclonal Antibody (HV735014) is a rabbit polyclonal antibody detecting EBF1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Danio rerio.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactivité des espèces

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human EBF1 (Asp179-Ser451).

Cible

EBF, EBF1, OE-1, COE1, Early B-cell factor, Transcription factor COE1, O/E-1

Purification

Purified by antigen affinity column.

Numéro d'accès

Q9UH73

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Transcription factor COE1 (EBF1) is a ~64 kDa protein. Key pioneer transcription factor of B-cell specification and commitment. Recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'. Operates in a transcription factor network to activate B-cell-specific genes and repress genes associated with alternative cell fates. For instance, positively regulates many B-cell specific genes including BCR or CD40 while repressing genes that direct cells into alternative lineages, including GATA3 and TCF7 for the T-cell lineage. In addition to its role during lymphopoiesis, controls the thermogenic gene program in adipocytes during development and in response to environmental cold.

1. Yang, CY. et al. (2016) Genes & development 30, 2310-2324. PMID: 27807034

Note

For research use only.

Images

Reférences

Fiche technique

Anti-EBF1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

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