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Anti-EBV/HHV4 BMRF1 Polyclonal Antibody (VK074014)

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Vista general
Número de catálogoVK074014
Descripción
Anti-EBV/HHV4 BMRF1 Polyclonal Antibody (VK074014) is a rabbit polyclonal antibody detecting DNA polymerase processivity factor BMRF1 in ELISA, IHC, WB.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactividad de especiesEpstein-Barr virus (HHV-4) (Human herpesvirus 4)
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant EBV/HHV4 BMRF1 (Met1-Ser314).
Objetivo DNA polymerase processivity factor BMRF1, Early Antigen Diffused, EA-D, Polymerase accessory subunit
Nivel de endotoxinas Please contact with the lab for this information.
Purificación Purified by antigen affinity column.
Número de acceso P03191
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

DNA polymerase processivity factor BMRF1 is a ~43 kDa protein. Acts as a DNA polymerase processivity factor; a transcriptional activator for several EBV promoters and inhibits the host DNA damage response (DDR) to double-stranded DNA breaks. Plays an essential role in the viral lytic DNA replication by acting as a polymerase accessory subunit. Stimulates the viral DNA polymerase activity and appears to function with it as a holoenzyme. Increases the processivity of the viral polymerase, probably by acting as a sliding clamp that prevents dissociation of the polymerase from the active template. In addition, BMRF1 transcriptionally activates the oriLyt early BHLF1 promoter.

1. Neuhierl, B. et al. (2006) Journal of virology 80, 5078-81. PMID: 16641300
2. Murayama, K. et al. (2009) The Journal of biological chemistry 284, 35896-905. PMID: 19801550
3. Salamun, SG. et al. (2019) Journal of virology 93. PMID: 31462557
4. Zhang, Q. et al. (1996) Journal of virology 70, 5131-42. PMID: 8764021
5. Zhang, Q. et al. (1997) Virology 230, 22-34. PMID: 9126259
Nota For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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