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Anti-EBV/HHV4 BMRF1 Polyclonal Antibody (VK074014)

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Aperçu
Numéro de catalogueVK074014
Description
Anti-EBV/HHV4 BMRF1 Polyclonal Antibody (VK074014) is a rabbit polyclonal antibody detecting DNA polymerase processivity factor BMRF1 in ELISA, IHC, WB.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesEpstein-Barr virus (HHV-4) (Human herpesvirus 4)
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant EBV/HHV4 BMRF1 (Met1-Ser314).
Cible DNA polymerase processivity factor BMRF1, Early Antigen Diffused, EA-D, Polymerase accessory subunit
Niveau d'endotoxines Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Numéro d'accès P03191
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

DNA polymerase processivity factor BMRF1 is a ~43 kDa protein. Acts as a DNA polymerase processivity factor; a transcriptional activator for several EBV promoters and inhibits the host DNA damage response (DDR) to double-stranded DNA breaks. Plays an essential role in the viral lytic DNA replication by acting as a polymerase accessory subunit. Stimulates the viral DNA polymerase activity and appears to function with it as a holoenzyme. Increases the processivity of the viral polymerase, probably by acting as a sliding clamp that prevents dissociation of the polymerase from the active template. In addition, BMRF1 transcriptionally activates the oriLyt early BHLF1 promoter.

1. Neuhierl, B. et al. (2006) Journal of virology 80, 5078-81. PMID: 16641300
2. Murayama, K. et al. (2009) The Journal of biological chemistry 284, 35896-905. PMID: 19801550
3. Salamun, SG. et al. (2019) Journal of virology 93. PMID: 31462557
4. Zhang, Q. et al. (1996) Journal of virology 70, 5131-42. PMID: 8764021
5. Zhang, Q. et al. (1997) Virology 230, 22-34. PMID: 9126259
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-EBV/HHV4 BMRF1 Polyclonal Antibody

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