Anti-EBV/HHV4 EBNA1/BKRF1 Polyclonal Antibody (VK686014)

Name: Anti-EBV/HHV4 EBNA1/BKRF1 Polyclonal Antibody
Brand: abinScience
SKU: VK686014
Price: 168 USD
Availability: InStock

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Prix(USD) :

Spécification :

50ug
100ug

Nombre :

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SDS

Aperçu

Numéro de catalogue

VK686014

Description

Anti-EBV/HHV4 EBNA1/BKRF1 Polyclonal Antibody (VK686014) is a rabbit polyclonal antibody detecting EBNA1 in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactivité des espèces

Epstein-Barr virus (HHV-4) (Human herpesvirus 4)

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant EBV/HHV4 EBNA1/BKRF1 (His468-Pro607).

Cible

Epstein-Barr nuclear antigen 1, EBNA-1, EBV nuclear antigen 1, EBNA1, BKRF1

Purification

Purified by antigen affinity column.

Numéro d'accès

P03211

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Epstein-Barr nuclear antigen 1 (EBNA1/BKRF1) is a ~56 kDa protein. Responsible for the origin of replication (oriP) dependent replication and maintenance of viral episomes during latent infection. EBNA1 dimer interacts with the DS (dyad symmetry) element within the origin of replication oriP and with a host mitotic chromosome to initiate viral DNA replication during latency. EBNA1 binding to DS recruits the host origin recognition complex (ORC). Governs the faithful mitotic segregation of the viral episomes by binding both the FR (family of repeats) element within oriP and the host mitotic chromosomes. Forms a cell cycle-dependent tyrosine-dependent DNA cross-link and single-strand cleavage at oriP required for terminating replication and maintaining viral episomes.

1. Sears, J. et al. (2004) Journal of virology 78, 11487-505. PMID: 15479791

2. Rawlins, DR. et al. (1985) Cell 42, 859-68. PMID: 2996781

3. Hodin, TL. et al. (2013) Journal of virology 87, 13020-8. PMID: 24067969

4. Malecka, KA. et al. (2019) Journal of virology 93. PMID: 31142669

5. Summers, H. et al. (1996) Journal of virology 70, 1228-31. PMID: 8551585

6. Ritzi, M. et al. (2003) Journal of cell science 116, 3971-84. PMID: 12953058

7. Hung, SC. et al. (2001) Proceedings of the National Academy of Sciences of the United States of America 98, 1865-70. PMID: 11172042

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-EBNA1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant EBV/HHV4 EBNA1/BKRF1 (Catalog No: VK686012)

Reférences

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