Anti-EDN1/Endothelin1 Polyclonal Antibody (HY182014)

Name: Anti-EDN1/Endothelin1 Polyclonal Antibody
Brand: abinScience
SKU: HY182014
Price: 168 USD
Availability: InStock

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Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HY182014

Description

Anti-EDN1/Endothelin1 Polyclonal Antibody (HY182014) is a rabbit polyclonal antibody detecting Endothelin1 in ELISA, IHC, WB. Suitable for Human and Macaca fascicularis.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactivité des espèces

Human

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human EDN1 (Ala18-Trp212).

Cible

Endothelin-1, EDN1, PPET1, ET-1, Preproendothelin-1, Endothelin1

Purification

Purified by antigen affinity column.

Numéro d'accès

P05305

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Endothelin-1 (EDN1) is a ~24 kDa protein. Endothelins are endothelium-derived vasoconstrictor peptides. Probable ligand for G-protein coupled receptors EDNRA and EDNRB which activates PTK2B, BCAR1, BCAR3 and, GTPases RAP1 and RHOA cascade in glomerular mesangial cells. Also binds the DEAR/FBXW7-AS1 receptor. Promotes mesenteric arterial wall remodeling via activation of ROCK signaling and subsequent colocalization of NFATC3 with F-actin filaments. NFATC3 then translocates to the nucleus where it subsequently promotes the transcription of the smooth muscle hypertrophy and differentiation marker ACTA2.

1. Rufanova, VA. et al. (2009) Journal of cellular physiology 219, 45-56. PMID: 19086031

2. Glorioso, N. et al. (2007) Circulation research 100, 1522-9. PMID: 17446437

Note

For research use only.

Images

Reférences

Fiche technique

Anti-EDN1/Endothelin1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

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