Anti-EEEV Spike glycoprotein E2 Polyclonal Antibody (VK634014)

Name: Anti-EEEV Spike glycoprotein E2 Polyclonal Antibody
Brand: abinScience
SKU: VK634014
Price: 168 USD
Availability: InStock

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Precio(USD):

Especificación:

50ug
100ug

Número:

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Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

VK634014

Descripción

Anti-EEEV Spike glycoprotein E2 Polyclonal Antibody (VK634014) is a rabbit polyclonal antibody detecting Spike glycoprotein E2 in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactividad de especies

Eastern equine encephalitis virus (EEEV)

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant EEEV Spike glycoprotein E2 (Asp323-Arg681).

Objetivo

Structural polyprotein, p130, Capsid protein, 3.4.21.90, Coat protein, C, Precursor of protein E3/E2, p62, pE2, Assembly protein E3, Spike glycoprotein E2, E2 envelope glycoprotein, 6K protein, Spike glycoprotein E1, E1 envelope glycoprotein

Purificación

Purified by antigen affinity column.

Número de acceso

P08768

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Structural polyprotein (Spike glycoprotein E2) is a ~137 kDa protein. Forms an icosahedral capsid with a T=4 symmetry composed of 240 copies of the capsid protein surrounded by a lipid membrane through which penetrate 80 spikes composed of trimers of E1-E2 heterodimers. The capsid protein binds to the viral RNA genome at a site adjacent to a ribosome binding site for viral genome translation following genome release. Possesses a protease activity that results in its autocatalytic cleavage from the nascent structural protein. Following its self-cleavage, the capsid protein transiently associates with ribosomes, and within several minutes the protein binds to viral RNA and rapidly assembles into icosahedric core particles. The resulting nucleocapsid eventually associates with the cytoplasmic domain of the spike glycoprotein E2 at the cell membrane, leading to budding and formation of mature virions.

1. Adams, LJ. et al. (2024) Cell 187, 360-374.e19. PMID: 38176410

Nota

For research use only.

Imágenes

Western blot

Western blot analysis was performed using anti-EEEV-E2 polyclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant EEEV Spike glycoprotein E2 (Catalog No: VK634012)

Referencias

Datasheet