Anti-EEEV Spike glycoprotein E2 Polyclonal Antibody (VK634014)

Name: Anti-EEEV Spike glycoprotein E2 Polyclonal Antibody
Brand: abinScience
SKU: VK634014
Price: 168 USD
Availability: InStock

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Spécification :

50ug
100ug

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SDS

Aperçu

Numéro de catalogue

VK634014

Description

Anti-EEEV Spike glycoprotein E2 Polyclonal Antibody (VK634014) is a rabbit polyclonal antibody detecting Spike glycoprotein E2 in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactivité des espèces

Eastern equine encephalitis virus (EEEV)

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant EEEV Spike glycoprotein E2 (Asp323-Arg681).

Cible

Structural polyprotein, p130, Capsid protein, 3.4.21.90, Coat protein, C, Precursor of protein E3/E2, p62, pE2, Assembly protein E3, Spike glycoprotein E2, E2 envelope glycoprotein, 6K protein, Spike glycoprotein E1, E1 envelope glycoprotein

Purification

Purified by antigen affinity column.

Numéro d'accès

P08768

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Structural polyprotein (Spike glycoprotein E2) is a ~137 kDa protein. Forms an icosahedral capsid with a T=4 symmetry composed of 240 copies of the capsid protein surrounded by a lipid membrane through which penetrate 80 spikes composed of trimers of E1-E2 heterodimers. The capsid protein binds to the viral RNA genome at a site adjacent to a ribosome binding site for viral genome translation following genome release. Possesses a protease activity that results in its autocatalytic cleavage from the nascent structural protein. Following its self-cleavage, the capsid protein transiently associates with ribosomes, and within several minutes the protein binds to viral RNA and rapidly assembles into icosahedric core particles. The resulting nucleocapsid eventually associates with the cytoplasmic domain of the spike glycoprotein E2 at the cell membrane, leading to budding and formation of mature virions.

1. Adams, LJ. et al. (2024) Cell 187, 360-374.e19. PMID: 38176410

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-EEEV-E2 polyclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant EEEV Spike glycoprotein E2 (Catalog No: VK634012)

Reférences

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