Anti-EEEV Spike glycoprotein E2 Polyclonal Antibody (VK634014)

Name: Anti-EEEV Spike glycoprotein E2 Polyclonal Antibody
Brand: abinScience
SKU: VK634014
Price: 168 USD
Availability: InStock

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Спецификация:

50ug
100ug

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Обзор

Каталожный номер

VK634014

Описание

Anti-EEEV Spike glycoprotein E2 Polyclonal Antibody (VK634014) is a rabbit polyclonal antibody detecting Spike glycoprotein E2 in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Реактивность видов

Eastern equine encephalitis virus (EEEV)

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant EEEV Spike glycoprotein E2 (Asp323-Arg681).

Цель

Structural polyprotein, p130, Capsid protein, 3.4.21.90, Coat protein, C, Precursor of protein E3/E2, p62, pE2, Assembly protein E3, Spike glycoprotein E2, E2 envelope glycoprotein, 6K protein, Spike glycoprotein E1, E1 envelope glycoprotein

Очистка

Purified by antigen affinity column.

Номер доступа

P08768

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

Structural polyprotein (Spike glycoprotein E2) is a ~137 kDa protein. Forms an icosahedral capsid with a T=4 symmetry composed of 240 copies of the capsid protein surrounded by a lipid membrane through which penetrate 80 spikes composed of trimers of E1-E2 heterodimers. The capsid protein binds to the viral RNA genome at a site adjacent to a ribosome binding site for viral genome translation following genome release. Possesses a protease activity that results in its autocatalytic cleavage from the nascent structural protein. Following its self-cleavage, the capsid protein transiently associates with ribosomes, and within several minutes the protein binds to viral RNA and rapidly assembles into icosahedric core particles. The resulting nucleocapsid eventually associates with the cytoplasmic domain of the spike glycoprotein E2 at the cell membrane, leading to budding and formation of mature virions.

1. Adams, LJ. et al. (2024) Cell 187, 360-374.e19. PMID: 38176410

Примечание

For research use only.

Изображения

Western blot

Western blot analysis was performed using anti-EEEV-E2 polyclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant EEEV Spike glycoprotein E2 (Catalog No: VK634012)

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