Anti-Human EGFR Polyclonal Antibody (HF004034)

Name: Anti-Human EGFR Polyclonal Antibody
Brand: abinScience
SKU: HF004034
Price: 168 USD
Availability: InStock

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100ug

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Fiche technique
SDS

Aperçu

Numéro de catalogue

HF004034

Description

Anti-EGFR/ERBB1/HER1 Polyclonal Antibody (HF004034) is a rabbit polyclonal antibody detecting ERBB1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactivité des espèces

Human, Mouse

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human EGFR/ERBB1/HER1 (Leu25-Ser151).

Cible

EC:2.7.10.1, EGFR, ERBB, ERBB1, Epidermal growth factor receptor, HER1, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1

Niveau d'endotoxines

Please contact with the lab for this information.

Pureté

>90% as determined by SDS-PAGE.

Purification

Purified by antigen affinity column.

Numéro d'accès

P00533

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Epidermal growth factor receptor (EGFR/ERBB1) is a ~134 kDa protein. Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. EGFR is the therapeutic target of cetuximab (Erbitux) and panitumumab (Vectibix).

1. Arcaro, A. et al. (2000) Molecular and cellular biology 20, 3817-30. PMID: 10805725

2. Runkle, KB. et al. (2016) Molecular cell 62, 385-396. PMID: 27153536

3. Chen, WS. et al. (1989) Cell 59, 33-43. PMID: 2790960

4. Bu, J. et al. (2022) Acta biochimica et biophysica Sinica 54, 400-408. PMID: 35538033

5. Garrett, TP. et al. (2002) Cell 110, 763-73. PMID: 12297049

6. Kochupurakkal, BS. et al. (2005) The Journal of biological chemistry 280, 8503-12. PMID: 15611079

7. Kim, J. et al. (2007) Cancer research 67, 9229-37. PMID: 17909029

8. Lu, C. et al. (2010) Molecular and cellular biology 30, 5432-43. PMID: 20837704

Note

For research use only.

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