Anti-EGLN3 Polyclonal Antibody (HP404014)

Name: Anti-EGLN3 Polyclonal Antibody
Brand: abinScience
SKU: HP404014
Price: 168 USD
Availability: InStock

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50ug
100ug

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Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HP404014

Reactivité des espèces

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human EGLN3 (Met1-Asp239).

Cible

PHD3, Hypoxia-inducible factor prolyl hydroxylase 3, Prolyl hydroxylase EGLN3, HPH-3, Prolyl hydroxylase domain-containing protein 3, Egl nine homolog 3, HIF-PH3, HIF-prolyl hydroxylase 3, HPH-1, EGLN3

Purification

Purified by antigen affinity column.

Numéro d'accès

Q9H6Z9

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Prolyl hydroxylase EGLN3 is a ~27 kDa protein. Prolyl hydroxylase that mediates hydroxylation of proline residues in target proteins, such as PKM, TELO2, ATF4, GPX4 and HIF1A. Target proteins are preferentially recognized via a LXXLAP motif. Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A.

1. Xie, L. et al. (2009) Science signaling 2, ra33. PMID: 19584355

2. Su, Y. et al. (2010) British journal of cancer 103, 1571-9. PMID: 20978507

3. Chen, N. et al. (2011) Cell research 21, 983-6. PMID: 21483450

4. Yan, B. et al. (2011) Biochemical and biophysical research communications 409, 315-20. PMID: 21575608

5. Luo, W. et al. (2011) Cell 145, 732-44. PMID: 21620138

6. Xie, L. et al. (2012) The Journal of clinical investigation 122, 2827-36. PMID: 22797300

7. Zheng, P. et al. (2025) Nature chemical biology 21, 1420-1432. PMID: 40281343

8. Epstein, AC. et al. (2001) Cell 107, 43-54. PMID: 11595184

9. Huang, J. et al. (2002) The Journal of biological chemistry 277, 39792-800. PMID: 12181324

Note

For research use only.

Images

Reférences

Fiche technique

Anti-EGLN3 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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