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Anti-ENO3 Polyclonal Antibody (HB156014)

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Anti-ENO3 Polyclonal Antibody
Anti-ENO3 Polyclonal Antibody
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Aperçu
Numéro de catalogueHB156014
Description
Anti-ENO3 Polyclonal Antibody (HB156014) is a rabbit polyclonal antibody detecting ENO3 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Salmo salar.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human ENO3 (Met1-Gly391).
Cible 2-phospho-D-glycerate hydro-lyase, Muscle-specific enolase, Skeletal muscle enolase, ENO3, Enolase 3, Beta-enolase, MSE
Purification Purified by antigen affinity column.
Numéro d'accès P13929
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Beta-enolase (ENO3) is a ~46 kDa protein. Enolase that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in glycolysis and the reverse reaction in gluconeogenesis. Appears to have a function in striated muscle development and regeneration.

Note For research use only.
Images
  • Anti-ENO3 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-ENO3 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: SH-SY5Y cell lysate
    Lane 2: Hela cell lysate
    Lane 3: HepG2 cell lysate
    Lane 4: Jurkat cell lysate
    Lane 5: A549 cell lysate
    Lane 6: Mouse brain lysate
    Lane 7: Mouse heart lysate
    Lane 8: Mouse skeletal muscle lysate
    Lane 9: Rat brain lysate
    Lane 10: Rat heart lysate
    Lane11: Rat skeletal muscle lysate

Reférences
Fiche technique
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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Anti-ENO3 Polyclonal Antibody

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