Anti-ERN1 Polyclonal Antibody (HF537014)

Name: Anti-ERN1 Polyclonal Antibody
Brand: abinScience
SKU: HF537014
Price: 168 USD
Availability: InStock

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Número de catálogo

HF537014

Descripción

Anti-ERN1 Polyclonal Antibody (HF537014) is a rabbit polyclonal antibody detecting ERN1 in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactividad de especies

Human, Mouse

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human ERN1 (Phe571-Leu977).

Objetivo

Endoplasmic reticulum-to-nucleus signaling 1, IRE1a, ERN1, Ire1-alpha, hIRE1p, Serine/threonine-protein kinase/endoribonuclease IRE1, Inositol-requiring protein 1, IRE1

Purificación

Purified by antigen affinity column.

Número de acceso

O75460

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Serine/threonine-protein kinase/endoribonuclease IRE1 (ERN1) is a ~109 kDa protein. Serine/threonine-protein kinase and endoribonuclease that acts as a key sensor for the endoplasmic reticulum unfolded protein response (UPR). In unstressed cells, the endoplasmic reticulum luminal domain is maintained in its inactive monomeric state by binding to the endoplasmic reticulum chaperone HSPA5/BiP. Accumulation of misfolded proteins in the endoplasmic reticulum causes release of HSPA5/BiP, allowing the luminal domain to homodimerize, promoting autophosphorylation of the kinase domain and subsequent activation of the endoribonuclease activity. The endoribonuclease activity is specific for XBP1 mRNA and excises 26 nucleotides from XBP1 mRNA. The resulting spliced transcript of XBP1 encodes a transcriptional activator protein that up-regulates expression of UPR target genes.

1. Iwawaki, T. et al. (2001) Nature cell biology 3, 158-64. PMID: 11175748

2. Yoshida, H. et al. (2001) Cell 107, 881-91. PMID: 11779464

3. Liu, CY. et al. (2003) The Journal of biological chemistry 278, 17680-7. PMID: 12637535

4. Lisbona, F. et al. (2009) Molecular cell 33, 679-91. PMID: 19328063

5. Ali, MM. et al. (2011) The EMBO journal 30, 894-905. PMID: 21317875

6. Liu, J. et al. (2017) Nature communications 8, 14186. PMID: 28128204

7. Chang, TK. et al. (2018) Molecular cell 71, 629-636.e5. PMID: 30118681

8. Kovaleva, V. et al. (2023) Cell reports 42, 112066. PMID: 36739529

9. Tirasophon, W. et al. (1998) Genes & development 12, 1812-24. PMID: 9637683

10. Eletto, D. et al. (2014) Molecular cell 53, 562-576. PMID: 24508390

Nota

For research use only.

Imágenes

Referencias

Datasheet

Anti-ERN1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo

Anti-ERN1 Polyclonal Antibody (HF537014)

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Referencias

Datasheet

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