Anti-ERN1 Polyclonal Antibody (HF537014)

Name: Anti-ERN1 Polyclonal Antibody
Brand: abinScience
SKU: HF537014
Price: 168 USD
Availability: InStock

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100ug

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Aperçu

Numéro de catalogue

HF537014

Description

Anti-ERN1 Polyclonal Antibody (HF537014) is a rabbit polyclonal antibody detecting ERN1 in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactivité des espèces

Human, Mouse

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human ERN1 (Phe571-Leu977).

Cible

Endoplasmic reticulum-to-nucleus signaling 1, IRE1a, ERN1, Ire1-alpha, hIRE1p, Serine/threonine-protein kinase/endoribonuclease IRE1, Inositol-requiring protein 1, IRE1

Purification

Purified by antigen affinity column.

Numéro d'accès

O75460

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Serine/threonine-protein kinase/endoribonuclease IRE1 (ERN1) is a ~109 kDa protein. Serine/threonine-protein kinase and endoribonuclease that acts as a key sensor for the endoplasmic reticulum unfolded protein response (UPR). In unstressed cells, the endoplasmic reticulum luminal domain is maintained in its inactive monomeric state by binding to the endoplasmic reticulum chaperone HSPA5/BiP. Accumulation of misfolded proteins in the endoplasmic reticulum causes release of HSPA5/BiP, allowing the luminal domain to homodimerize, promoting autophosphorylation of the kinase domain and subsequent activation of the endoribonuclease activity. The endoribonuclease activity is specific for XBP1 mRNA and excises 26 nucleotides from XBP1 mRNA. The resulting spliced transcript of XBP1 encodes a transcriptional activator protein that up-regulates expression of UPR target genes.

1. Iwawaki, T. et al. (2001) Nature cell biology 3, 158-64. PMID: 11175748

2. Yoshida, H. et al. (2001) Cell 107, 881-91. PMID: 11779464

3. Liu, CY. et al. (2003) The Journal of biological chemistry 278, 17680-7. PMID: 12637535

4. Lisbona, F. et al. (2009) Molecular cell 33, 679-91. PMID: 19328063

5. Ali, MM. et al. (2011) The EMBO journal 30, 894-905. PMID: 21317875

6. Liu, J. et al. (2017) Nature communications 8, 14186. PMID: 28128204

7. Chang, TK. et al. (2018) Molecular cell 71, 629-636.e5. PMID: 30118681

8. Kovaleva, V. et al. (2023) Cell reports 42, 112066. PMID: 36739529

9. Tirasophon, W. et al. (1998) Genes & development 12, 1812-24. PMID: 9637683

10. Eletto, D. et al. (2014) Molecular cell 53, 562-576. PMID: 24508390

Note

For research use only.

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Reférences

Fiche technique

Anti-ERN1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

Kits d'Essai

Anti-ERN1 Polyclonal Antibody (HF537014)

Aperçu

Images

Reférences

Fiche technique

SDS