中文
English
한국어
日本語
Español
Français
Русский
| カタログ番号 | HF537014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-ERN1 Polyclonal Antibody (HF537014) is a rabbit polyclonal antibody detecting ERN1 in ELISA, IHC, WB. Suitable for Human and Mouse.
Highlights
|
||||||||
| 種反応性 | Human, Mouse | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human ERN1 (Phe571-Leu977). | ||||||||
| ターゲット | Endoplasmic reticulum-to-nucleus signaling 1, IRE1a, ERN1, Ire1-alpha, hIRE1p, Serine/threonine-protein kinase/endoribonuclease IRE1, Inositol-requiring protein 1, IRE1 | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | O75460 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
||||||||
| 製品使用情報 |
| ||||||||
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Serine/threonine-protein kinase/endoribonuclease IRE1 (ERN1) is a ~109 kDa protein. Serine/threonine-protein kinase and endoribonuclease that acts as a key sensor for the endoplasmic reticulum unfolded protein response (UPR). In unstressed cells, the endoplasmic reticulum luminal domain is maintained in its inactive monomeric state by binding to the endoplasmic reticulum chaperone HSPA5/BiP. Accumulation of misfolded proteins in the endoplasmic reticulum causes release of HSPA5/BiP, allowing the luminal domain to homodimerize, promoting autophosphorylation of the kinase domain and subsequent activation of the endoribonuclease activity. The endoribonuclease activity is specific for XBP1 mRNA and excises 26 nucleotides from XBP1 mRNA. The resulting spliced transcript of XBP1 encodes a transcriptional activator protein that up-regulates expression of UPR target genes. 1. Iwawaki, T. et al. (2001) Nature cell biology 3, 158-64. PMID: 11175748 2. Yoshida, H. et al. (2001) Cell 107, 881-91. PMID: 11779464 3. Liu, CY. et al. (2003) The Journal of biological chemistry 278, 17680-7. PMID: 12637535 4. Lisbona, F. et al. (2009) Molecular cell 33, 679-91. PMID: 19328063 5. Ali, MM. et al. (2011) The EMBO journal 30, 894-905. PMID: 21317875 6. Liu, J. et al. (2017) Nature communications 8, 14186. PMID: 28128204 7. Chang, TK. et al. (2018) Molecular cell 71, 629-636.e5. PMID: 30118681 8. Kovaleva, V. et al. (2023) Cell reports 42, 112066. PMID: 36739529 9. Tirasophon, W. et al. (1998) Genes & development 12, 1812-24. PMID: 9637683 10. Eletto, D. et al. (2014) Molecular cell 53, 562-576. PMID: 24508390 | ||||||||
| 注意事項 | For research use only. |
お問い合わせ
+86-027-65523339
中国武漢市深敦寺路666号C棟、武漢、430206
