Anti-Escherichia coli O157:H7 shiga toxin type 1 B/stx1B Recombinant Antibody (5-5B) (JN072013)

Name: Anti-Escherichia coli O157:H7 shiga toxin type 1 B/stx1B Recombinant Antibody (5-5B)
Brand: abinScience
SKU: JN072013
Price: 126 USD
Availability: InStock

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Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

JN072013

Reactivité des espèces

Escherichia coli

Applications

ELISA, FCM, WB

Espèce hôte

Mouse

Clonalité

Monoclonal

Isotype

IgG2a

ID de clone

5-5B

Cible

stx1, stx1B, Shiga toxin 1 B subunit, Shiga toxin I B subunit, Escherichia coli O157:H7

Niveau d'endotoxines

Please contact with the lab for this information.

Pureté

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Numéro d'accès

Q7BQ98, Q32GM0, P69178, P69179, Q779K3

RRID

Anti-Escherichia coli O157:H7 shiga toxin type 1 B/stx1B Antibody (5-5B) (abinScience Cat# JN072013, RRID:AB_3729026)
_x005F_x005F_x000D_

Forme

Liquid

Tampon de stockage

0.01M PBS pH 7.4

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Contexte

Shiga toxin subunit B (shiga toxin type 1B/stx1B) is a ~9 kDa protein. The B subunit is responsible for the binding of the holotoxin to specific receptors on the target cell surface, such as globotriaosylceramide (Gb3) in human intestinal microvilli.

Note

For research use only.

Images

Bioactivity

SEC-HPLC detection for Anti-Escherichia coli O157:H7 shiga toxin type 1 B/stx1B Antibody (5-5B).
Western blot

Western blot analysis was performed using anti-stx1B monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant E. coli stx1B (Catalog No: JN072012)
Lane 2: negative control
Bioactivity

Detects recombinant E. coli shiga toxin type 1 B/stx1B (Catalog No: JN072012) in indirect ELISA

Reférences

Fiche technique

Anti-Escherichia coli O157:H7 shiga toxin type 1 B/stx1B Recombinant Antibody (5-5B).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

Kits d'Essai