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Anti-Escherichia coli ompA Recombinant Nanobody (SAA2320) (JN116013)

Anti-Escherichia coli ompA Recombinant Nanobody (SAA2320)
Anti-Escherichia coli ompA Recombinant Nanobody (SAA2320)
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Aperçu
Numéro de catalogueJN116013
Description
Anti-Escherichia coli ompA Nanobody (SAA2320) [SAA2320] (JN116013) is a human monoclonal antibody detecting OmpA in ELISA, FCM. Suitable for Escherichia coli.
Highlights
  • ●Flow Cytometry — Validated for flow cytometry applications.
Reactivité des espècesEscherichia coli
ApplicationsELISA, FCM
Espèce hôteHuman
ClonalitéMonoclonal
IsotypeVHH-Fc
ID de cloneSAA2320
Cible Outer membrane protein A, OmpA, Outer membrane porin A, Outer membrane protein 3A, Outer membrane protein B, Outer membrane protein II*, Outer membrane protein d, ompA, con, tolG, tut
Niveau d'endotoxines Please contact with the lab for this information.
Pureté >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Numéro d'accès P0A910, P0A911
Forme Liquid
Tampon de stockage 0.01M PBS pH 7.4

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:2000-1:20000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Outer membrane protein A (ompA) is a ~37 kDa protein. With TolR probably plays a role in maintaining the position of the peptidoglycan cell wall in the periplasm (Probable). Plays a role in resistance to environmental stress, and a role in outer membrane functionality and cell shape. Non-covalently binds peptidoglycan (Probable). Acts as a porin with low permeability that allows slow penetration of small solutes. A very abundant protein, there can be up to 210,000 OmpA molecules per cell.

1. Wang, Y. (2002) Biochemical and biophysical research communications 292, 396-401. PMID: 11906175
2. Sonntag, I. et al. (1978) Journal of bacteriology 136, 280-5. PMID: 361695
3. Ishida, H. et al. (2014) Biochimica et biophysica acta 1838, 3014-24. PMID: 25135663
4. Sugawara, E. et al. (1992) The Journal of biological chemistry 267, 2507-11. PMID: 1370823
5. Negoda, A. et al. (2010) Biochimica et biophysica acta 1798, 1480-4. PMID: 20004640
6. Negoda, A. et al. (2010) The FEBS journal 277, 4427-37. PMID: 21069910
7. Li, GW. et al. (2014) Cell 157, 624-35. PMID: 24766808
8. Sugawara, E. et al. (1994) The Journal of biological chemistry 269, 17981-7. PMID: 7517935
9. Arora, A. et al. (2000) The Journal of biological chemistry 275, 1594-600. PMID: 10636850
10. Zakharian, E. et al. (2005) Biochemistry 44, 6701-7. PMID: 15850404
Note For research use only
Images
  • Anti-Escherichia coli ompA Recombinant Nanobody (SAA2320)

    SDS-PAGE

    SDS-PAGE for Anti-Escherichia coli ompA Recombinant Nanobody (SAA2320)

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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