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Anti-ETFB Polyclonal Antibody (HW802014)

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Vista general
Número de catálogoHW802014
Descripción
Anti-ETFB Polyclonal Antibody (HW802014) is a rabbit polyclonal antibody detecting ETFB in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman, Mouse, Rat
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human ETFB (Ala2-Ile255).
Objetivo Beta-ETF, ETFB, Electron transfer flavoprotein subunit beta
Purificación Purified by antigen affinity column.
Número de acceso P38117
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Electron transfer flavoprotein subunit beta (ETFB) is a ~27 kDa protein. Heterodimeric electron transfer flavoprotein that accepts electrons from several mitochondrial dehydrogenases, including acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (Probable). Required for normal mitochondrial fatty acid oxidation and normal amino acid metabolism. ETFB binds an AMP molecule that probably has a purely structural role.

1. Toogood, HS. et al. (2004) The Journal of biological chemistry 279, 32904-12. PMID: 15159392
2. Toogood, HS. et al. (2005) The Journal of biological chemistry 280, 30361-6. PMID: 15975918
3. Małecki, J. et al. (2015) The Journal of biological chemistry 290, 423-34. PMID: 25416781
4. Olsen, RK. et al. (2003) Human mutation 22, 12-23. PMID: 12815589
5. Colombo, I. et al. (1994) Human molecular genetics 3, 429-35. PMID: 7912128
6. Roberts, DL. et al. (1996) Proceedings of the National Academy of Sciences of the United States of America 93, 14355-60. PMID: 8962055
Nota For research use only.
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Referencias
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-ETFB Polyclonal Antibody

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